The project plans to build a sequence-accurate commercial peptide bank, or parallel peptide bank, with a total of more than 175,000 peptides, named HuiKang International Peptide Bank(China Peptide Bank).
HuiKang International Peptide Bank(China Peptide Bank) contains a large number of unknown active epitope peptides, and will become a treasure house that can be shared all over the world. In addition to screening lead active epitope peptides for biomedical research, it can also provide active peptides for pharmaceutical, food, health care products, cosmetics, catalysts and materials industries. It is a basic and strategic project of biomedical research and development innovation in China and even in the world, which will make invaluable contributions to human life science, producing great social and economic benefits.
All kinds of physiological and biochemical activities are basically the result of mutual identification, interaction and balance between protein molecules. When the balance is out, disease appears. Therefore, by restoring the balance, diseases can be cured. Epitope peptides, a small molecule peptide composed of several key residues, contribute to most of the protein molecule binding energy. It is essential to discover epitope peptides for rebuilding the balance.
This recognition and binding, widely exists between cells, cells and stroma, stroma and stroma, which can be regarded as a unified receptor and ligand binding. Researchers found that the recognition and binding between two proteins is usually not the whole protein molecule, but the peptide containing three to five amino acids. This peptide consists of three to five amino acids is known as active epitope peptide. The discovery and screening of active epitope peptides is an important part in new drugs research.
Advantage one: half the effort with double the result, or even "one to quadruple", which greatly reduces the requirement for scientists.
In the past, screening active epitope peptides from natural peptide bank such as snake, bee and scorpion venom, or compound peptide bank such as protein degradation products, required five steps: isolation and purification; activity detection; sequence identification; synthesis and purification; and activity verification. The whole process usually requires at least four different professional scientists. However, China peptide bank only need to screen active epitope peptides through accurate parallel peptide bank. The screening method is simpler, efficiency is higher, and workload is less.
Advantage two: there is no quenching phenomenon between active substances, and the high-affinity epitope peptides could be obtained.
Chemical combination peptide bank and phage display peptide bank, high affinity peptides are likely to synthesize or express in pairs and quenched each other, and finally unable to obtain the most affinity active epitope peptides. China peptide bank has high content of active substances, accurate sequence, and no receptor ligand neutralization and quenching between active substances, which could be applied to a variety of screening platforms.
HuiKang International Peptide Bank(China Peptide Bank) adopts PD-1 - PD-L1 blocking peptide screening solution. The weak blocking peptide was screened first, and the peptide chain was extended. Then the strong blocking peptide was screened, extending peptide chain in order to improve the selectivity and specificity.
HuiKang International Peptide Bank(China Peptide Bank) contains 175,000 peptides, each of which likes a fish and is kept in separate tanks forming a huge fishing reservoir. Scientist's method of identifying activity is a hook, and if a fish bites, he can find the expected lead peptides.
HuiKang International Peptide Bank(China Peptide Bank) is a commercial peptide bank. Scientists in the field of biomedicine around the world can make up their own peptide bank by theoretical simulation screening, and then purchase directly completing the leading compound screening research.
A drug screening system could be formed by using HuiKang International Peptide Bank (China Peptide Bank) combining any activity testing method. If an identification method is automated, it constitutes a large-scale high-throughput rapid screening platform, which is not expensive. Basic research and computer simulation techniques also can be applied to roughly screen peptide bank. Eliminating peptides that are not likely to be active, and then "fishing" to substantially reduce costs.